Biochem/physiol Actions

Km = 19 µM as a fluorescence resonance energy transfer (FRET) peptide substrate for TACE

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetA fluorescence resonance energy transfer (FRET) peptide substrate for TACE

General description

A fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (K_m ~19 µM). Cleavage occurs between the alanine and valine residues. Useful for screening TACE inhibitors. Excitation max: ~340 nm; emission max: ~490 nm.

A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (K_m = 19 µM). Substrate cleavage occurs at the Ala-Val bond. Useful for the screening of TACE and SARS protease 3-CL^PRO inhibitors.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bacha, U., et al. 2004. Biochemistry43, 4906.Patel, I.R., et al. 1998. J. Immunol.160, 4570.

Packaging

Yes

500 µg in Plastic ampoule

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS

Warning

Toxicity: Standard Handling (A)